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March 14 2013
Pristionchus pacificus protocols updated in WormMethods
Pristionchus pacificus protocols has been revised and greatly expanded by Andre Pires da Silva.
In addition to updated protocols for freezing, mutagenesis, deletion libraries and gene knockdown in P. pacificus, the revised chapter includes
protocols for: in situ hybridization, isolating genomic DNA for whole genome sequencing, extracting compounds from worm supernatant,
electron microscopy, immunofluorescence, transgenesis, and mapping mutants with molecular markers.
February 22 2013
New Worm Breeder's Gazette published
Volume 19, #3 of The Worm Breeder's Gazette has just been published. In addition to
beautiful cover artwork by Greg Nelson and Feng Xue, this issue contains 18 articles as well as announcements of 5 new labs.
February 21 2013
Chapter on cell culture added to WormMethods
Cell isolation and culture by Sihui Zhang and Jeffrey Kuhn has been published in the Methods in cell biology section. This chapter provides comprehensive techniques and media recipes for large-scale isolation and culture of primary embryonic and larval cells from C. elegans.
January 18 2013
New chapter published in Biochemistry
Ascaroside signaling in C. elegans, by Andrease Ludewig and Frank Schroeder, has been added to the Biochemistry section. This chapter describes the function, structure, biosynthesis and receptors of these small signaling molecules.
January 17, 2013
Updated chapter added to Signal transduction
Notch signaling: genetics and structure by Iva Greenwald and Rhett Kovall, which updates LIN-12/Notch signaling in C. elegans by Iva Greenwald, has been published in the Signal transduction section. In addition to covering the genetics of Notch in C. elegans, the updated chapter describes how recent advances in the structural biology of Notch signaling have enhanced understanding of its molecular mechanism.
December 18, 2012
Feeding behavior chapter added to WormMethods
Methods for measuring pharyngeal behaviors, by David Raizen, Bo-mi Song, Nick Trojanowski and Youn-Jai You, has been published in the Behavior section of WormMethods. Protocols for measuring and analyzing pharyngeal activity such as feeding rate, the timing of pharyngeal movements and the cessation of feeding are provided.
December 13, 2012
smFISH protocol now available in WormMethods
Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos by Ni Ji and Alexander van Oudenaarden has just been published in the Gene expression section of WormMethods. The authors provide the necessary technical details to set up and perform smFISH, from sample preparation to data analysis.
November 30, 2012
New Chapter added to Sex determination
Morphogenesis of the vulva and the vulva-uterine connection by Bhagwati Gupta, Wendy Hanna-Rose and Paul Sternberg has been published in the Sex determination section. This chapter updates the information on vulva morphogenesis found in Vulva development and provides more in-depth discussion on this topic.
November 29, 2012
New chapter on Eph receptor signaling published
Eph receptor signaling in C. elegans by Michael A. Miller and Ian D. Chin-Sang is available in the Signal transduction section. This chapter describes advances in our understanding of the biological functions and transduction mechanisms of this important class of receptor protein-tyrosine kinases.
November 20, 2012
Updated chapter published in Biochemistry
Trans-splicing and operons in C. elegans by Thomas Blumenthal is available in the Biochemistry section of WormBook.
This is an update of Trans-splicing and operons, published in 2005. The updated chapter describes advances in our
understanding of the mechanism and evolution of trans-splicing, and the structure and function of operons in C. elegans.
September 13, 2012
Worm trackers described in WormMethods
Keeping track of worm trackers, by Steven Husson et al. has
been added to the Behavior section of WormMethods. This chapter
describes several methods for automated image capture and
quantitation of nematode locomotion.
July 6, 2012
New Worm Breeder's Gazette published
The latest issue, Volume 19, number 2, of The Worm Breeder's Gazette is now available. Please click here to read about new techniques, research advances, useful web resources and more. This issue also includes lists of notable recent papers in genetics, biochemistry and reproductive biology as well as announcements of new C. elegans labs.
May 22, 2012
New chapter published in Neurobiology and behavior
C. elegans feeding has been published in the Neurobiology and behavior section of WormBook. This chapter, by Leon Avery and Young-jai You, describes the mechanics of feeding, the actions of the neurons and muscles involved, and the effects of food on behavior.
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