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June 4 2013
Transcription regulation chapter updated in Molecular Biology
Transcriptional regulation of gene expression in C. elegans by Valerie Reinke, Michael Krause and Peter Okkema has been added to the
Molecular biology section. This chapter updates Transcriptional regulation by Peter Okkema and Michael Krause, and includes discussions
of gene organization and regulation, transcription factors and their targets, chromatin regulatory complexes,
and genome-scale analyses of transcription regulation. Sortable Excel lists of transcription factors and their targets are also provided.
March 14 2013
Pristionchus pacificus protocols updated in WormMethods
Pristionchus pacificus protocols has been revised and greatly expanded by Andre Pires da Silva.
In addition to updated protocols for freezing, mutagenesis, deletion libraries and gene knockdown in P. pacificus, the revised chapter includes
protocols for: in situ hybridization, isolating genomic DNA for whole genome sequencing, extracting compounds from worm supernatant,
electron microscopy, immunofluorescence, transgenesis, and mapping mutants with molecular markers.
February 22 2013
New Worm Breeder's Gazette published
Volume 19, #3 of The Worm Breeder's Gazette has just been published. In addition to
beautiful cover artwork by Greg Nelson and Feng Xue, this issue contains 18 articles as well as announcements of 5 new labs.
February 21 2013
Chapter on cell culture added to WormMethods
Cell isolation and culture by Sihui Zhang and Jeffrey Kuhn has been published in the Methods in cell biology section. This chapter provides comprehensive techniques and media recipes for large-scale isolation and culture of primary embryonic and larval cells from C. elegans.
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