Worm Breeder's Gazette 3(2): 16
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
For radioautographic studies of in vitro RNA synthesis during C. elegans oogenesis, J. Starck used M9 buffer (BRENNER, 1974) as an incubation medium. The results of labelling were rather good, they showed a mode of RNA synthesis which should correspond to a normal metabolism, (J. Starck, 1977). The longer incubation time was two hours. Electron microscopic studies showed that subcellular structures were not keep good we often remarked broken nuclear membranes and mitochondria as swelled nuclei. The oocytes seemed to be hypertrophied and parietal cells were crushed. These observations became more numerous when the incubation times became longer. Furthermore, systematic studies on fixatives showed that the best one has an osmotic pression around 400 mOsM the M9 buffer osmotic pression is only about 300 mOsM. We try to make better the incubation medium by adding saccharose. With 3% of this product, the osmotic pression of the medium, called M10, is about 400 mOsM. New essays with this M10 gave the same results in photonic radioautographies. In electron microscopic studies, we note a normal cellular ultrastructure, without accidents on nuclei or mitochondria.