Worm Breeder's Gazette 16(1): 62 (October 1, 1999)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

An Attempt to Slow Aging in C. elegans. 12. No Effect of Lidocaine.

Vladimir V. Bakaev, Elena M. Kozhina, Natali A. Astapova

Box 45, Novosibirsk, 630107, Russia E-mail: bakaev@online.nsk.su

The purpose of this study was to investigate the effect of different concentrations of lidocaine hydrochloride in water solutions on nematode life span. In this experiment lidocaine hydrochloride was used in following dilutions: 1:104, 1:105, 1:106 and 1:107.

Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without lidocaine hydrochloride) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with lidocaine hydrochloride in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.

Concentration of lidocaine hydrochloride n Longevity (days)mean + S.E. maximal

Control 36 13.1 + 0.7 26

1:104 36 13.1 + 0.5 21

1:105 36 12.9 + 0.7 23

1:106 36 14.0 + 0.7 24

1:107 36 12.3 + 0.6 19

Conclusion: If lidocaine hydrochloride solution was applied to C. elegans, it was not able to increase their mean as well as maximal longevity.

Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.