Worm Breeder's Gazette 14(4): 50 (October 1, 1996)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
box 45, Novosibirsk, 630107, USSR
The purpose of this study was the effect of different concentrations of royal jelly extract on nematode life span. In this experiment royal jelly extract was used in the form of "Apilactosa" (produced by "Fertane", Moscow, Nagatinskaya str., 1) in following dilutions : 1:10*3, 1:10*4, 1:10*5, 1:10*6 , 1:10*7 , 1:10*8 , 1:10*9 and 1:10*10. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without royal jelly extract) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with royal jelly extract in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Concentration of royal Longevity jelly extract n mean +/- S.D. maximal 1:10*3 12 Lethal 1:10*4 11 18,2 +/- 1,8 28 1:10*5 12 19,2 +/- 2,2 32 1:10*6 12 19,3 +/- 1,7 28 1:10*7 12 15,8 +/- 2,2 28 1:10*8 11 20,6 +/- 3,1 32 1:10*9 8 12,3 +/- 3,5 34 1:10*10 12 17,5 +/- 2,3 31 Conclusion: If the extract from royal jelly was applied to C. elegans during the whole life span in above described conditions, the most effective concentration was 1:10*8. It seemed for me more reasonable to study the effect on longevity of any concentrations near 1:10*8. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.